Interaction of biospecies with surfaces
In the last decade, the study and development of biosensing platforms aimed to early and in-trace bio-diagnostics induced the study of the biological research protocols and methodologies in order to adapt them to the specific solid substrates, core of the sensing platforms and asses readily-reproducible step-by-step procedures for biospecies handling, grafting and detection suitable the several biosensing experiments and signal transduction method.
The approach consists in:
a) the choice of surfactants, buffer composition, pH condition, etc. compatible with the material of the solid support, that is the biosensor, for the covalent grafting of the receptor (e.g. Antibody-proteinA/G oriented)/probe (e.g. ss-DNA) grafted on the surface thanks to the previous chemical functionalization. These media at the same time have to solvate the biospecies and to leave unaffected the substrate properties (roughness, chemistry, ecc., structural integrity).
b) the modification of standard biological assay techiques such as ELISA, ECL, Fluorescence for the quantification of grafted receptors/probe and control for bio-recognition target (antigens, miRNA, ssDNA, oligopeptides)
c) the sensors regeneration so allowing the re-use of the same chip/device only for the last step target detection without the need to repeated the chemical functionalization and the bioreceptors/probes grafting on a new item.
These procedure have been developed for Micro-cantilevers mass sensors for Angiopoietin (Tumoral Angiogenesis marker), b-estradiol, bacteria detection; 1D Photonic Crystals coupling Bloch Surface Waves for Angiopoietin detection (Tumoral Angiogenesis marker) and miRNA16, miRNA21 (breast and colon cancer markers) detection ; SERS substrates, based on Ag nanoparticle in-situ generated by Mesoporous Silicon membranes, for leaver tumoral antigenic markers and miRNA16, miRNA21, miRNA222, also for multiplexed arrayed sensing.
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