Electronic Biosensing

Organic Electrochemical Transistor (OECT)

 

OECTs are based on highly conductivity polymer as poly(3,4-ethylenedioxythiophene) polystyrene sulfonate PEDOT:PSS, which modulates its conductivity, in response to a gate voltage, by bulk effects of doping and de-doping in a electrolyte solution. This unique characteristic allows for obtaining sensors able to monitor, with high sensibility, biomolecules as DNA and proteins, bio-signals as neuronal synaptic activity and so on. Materials and processing of these devices are extremely interesting due to the simple deposition of the polymers on flexible substrates by clean room processes or printing technology. The research activity in this fields is focused on the development of innovative processes to obtain novel devices for bio sensing applications as biomarkers recognition, cell analysis, health care and environmental monitoring.

Contact information

Matteo Cocuzza
Tel. +39 011 090 7392
matteo.cocuzza@infm.polito.it

Simone Marasso
Tel. +39 011 911 4899
simone.marasso@polito.it

 

Graphene Field Effect Transistor (G-FET)

 

G-FET are transistors which exploit Graphene as a channel instead of the classical semiconductor. Due to its peculiar electrical properties, Graphene is perfectly suitable for sensing applications and can be functionalized in order to allow the detection of analytes, like antibodies, DNA, miRNA and so on. Its mechanical properties and stability make it an interesting material also for the production of flexible electronics, which are the basis for example of wearable devices.

In our research activity we are working on developing G-FETs for biosensing, specifically for the detection of lung cancer biomarkers. An important point of research is the transferring process of Graphene, which is one of the main issues for its exploitation on an industrial scale.

Contact information
 

Simone Marasso
Tel. +39 011 911 4899
simone.marasso@polito.it

Alberto Ballesio

alberto.ballesio@polito.it

Alessio Verna

Alessio.Verna@polito.it

 

Electrolyte Gated Organic Field Effect transistor (EGO-FET)

 

EGOFETs consist of an organic semiconducting material (OSC) separated from the gate electrode by an electrolyte and contacted with source and drain electrodes. The charge transport inside the OSC channel is controlled by the capacitive coupling between the electrical double layer (EDL) at the gate electrode/electrolyte interface and the one at the OSC/electrolyte interface. Small variations in these EDLs capacitances are able to modulate the drain current of the device, thus allowing for the detection of small molecules after the proper functionalization of the surface. The low operating voltages of EGOFETs and their easy processability have made these devices very attractive for low-cost point-of-care applications. Our research activity is focused on the development of EGOFETs based on polymeric semiconductors such as poly(3-hexylthiophene-2,5-diyl) (P3HT) for the detection of lung cancer biomarkers

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Contact information
 

Simone Marasso
Tel. +39 011 911 4899
simone.marasso@polito.it

Matteo Parmeggiani

matteo.parmeggiani@polito.it

Alessio Verna

Alessio.Verna@polito.it

 

Electrochemical based sensors

 

The basic components of an electrochemical sensor are the working electrode, the counter electrode and the reference electrode that are easily distinguished in the Screen Printed Electrodes (SPE). Using cleanroom approach we are able to produce different types of SPE designs on silicon and polymer surfaces. Changing the metal of the working and reference electrode is possible to measure a wide range of analytes from heavy metals to biomolecules using a portable potentiostat instrument. We are interested to scale the SPE design and develop new functionalization protocols to provide a multi sensor array for healthcare, cancer biomarkers and environmental monitoring.

Contact information

Giorgio Scordo

giorgio.scordo@polito.it

Alessio Verna

Alessio.Verna@polito.it

 

Publications

 

  1. “Nanogap fabrication for molecular electronics”, P. Civera, G. Piccinini, D. Demarchi, M. Cocuzza, D. Perrone, in: Book of Abstracts. Transalp'Nano 2008. Lyon. 27-29 October 2008. (pp. 100-101)
  2. "Liposomes sensing and monitoring by Organic Electrochemical Transistors integrated in microfluidics", G. Tarabella, A.G. Balducci, N. Coppedè, S. Marasso, S. Barbieri, M. Cocuzza, P. Colombo, R. Mosca, F. Sonvico, S. Iannotta, Biochimica et Biophysica Acta (BBA) - General Subjects, 1830, 2013, pp. 4374-4380, 10.1016/j.bbagen.2012.12.018
  3. "Biorecognition in organic field effect transistors biosensors: the role of the density of states  of the organic semiconductor", M. Berto, S. Casalini, M. Di Lauro, S. Marasso, M. Cocuzza,  D. Perrone, M. Pinti, A. Cossarizza, C.F. Pirri, D. Simon, M. Berggren, F. Zerbetto, C. Bortolotti, F. Biscarini, Analytical Chemistry, 2016, 88 (24), pp. 12330–12338, doi:10.1021/acs.analchem.6b03522
  4. "Monitoring the adaptive cell response to hyperosmotic stress by organic devices", P. D'Angelo, G. Tarabella, A. Romeo, A. Giodice, S. L. Marasso, M. Cocuzza, F. Ravanetti, A. Cacchioli, P. G. Petronini, S. Iannotta, MRS Communications, 2017, pp. 1-7, doi:10.1557/mrc.2017.29
  5. "Monitoring emulsion microstructure by using organic electrochemical transistors", Preziosi, V., Barra, M., Perazzo, A., Tarabella, G., Romeo, A., Marasso, S.L., D'Angelo, P., Iannotta, S., Cassinese, A., Guido, S., 2017, Journal of Materials Chemistry C, 5 (8), pp. 2056-2065
  6. M. Parmeggiani, A. Dev, P. Björk, and J. Linnros, “Electrokinetic-assisted gating in a microfluidic integrated Si nanoribbon ion sensor for enhanced sensitivity,” Sensors Actuators B Chem., vol. 262, pp. 974–981, Jun. 2018.
  7. G. Scordo, D. Moscone, G. Palleschi, and F. Arduini, “A reagent-free paper-based sensor embedded in a 3D printing device for cholinesterase activity measurement in serum,” Sensors Actuators B Chem., vol. 258, pp. 1015–1021, Apr. 2018.